Chaperonin



Chaperonin (CPN) are oligomeric proteins which mediate the folding of polypeptide chains. Group I CPNs are found in bacteria, chloroplasts and mitochondria. They include the most characterized GroEL/GroES complex from Escherichia coli and CPN60/CPN10 from Thermus thermophilus. The larger subunit (GroEL, CPN60) contains 3 domains. The apical domain is the one which binds the substrate. Group II CPNs are found in eukaryotic cytosol and archaea. Thermosome is a CPN complex found in archaea. CCT is a CPN complex found in eukarya.

3D Structures of Chaperonin
Update June 2011

Group I
Large subunit

3m6c – MtGroEL1 apical domain – Mycobacterium tuberculosis 1sjp – MtGroEL2 residues 42-539 3fbh, 2eu1, 1j4z, 1kpo, 1oel, 1grl, 2yey – EcGroEL (mutant) - Escherichia coli 3e76, 2nwc, 1xck, 1ss8 – EcGroEL 3c9v, 3cau – EcGroEL – Cryo EM 2c7e, 1gr5 - EcGroEL (mutant) – Cryo EM 1kp8 - EcGroEL (mutant) + ATP 1sx3 - EcGroEL + ATP 1sx4 - EcGroEL + ADP 1mnf – EcGroEL + polypeptide 2cgt – EcGroEL + capsid assbly protein GP31 – EM 1fy9, 1fya, 1kid – EcGroEL apical domain (mutant)  1srv – TtCPN60 apical domain - Thermus thermophilus 1iok – CPN60 – Paracoccus denitrificans

Small subunit

3nx6 – GroES residues 40-134 (mutant) – Xanthomonas oryzae 1wnr – TtCPN10 residues 1-94 1p3h, 1hx5 – MtCPN10 1p82, 1p83 - MtCPN10 residues 1-25 - NMR<BR /> 1egs - GroES residues 19-27 - NMR

Large + small subunit

1svt, 1pcq - EcGroEL + GroES<BR /> 1pf9, 1aon - EcGroEL + GroES + ADP<BR /> 2c7c, 2c7d - EcGroEL + GroES – Cryo EM<BR /> 1gru - EcGroEL + GroES + ATP + ADP – Cryo EM<BR /> 1we3 – TtCPN60 + CPN10<BR /> 1wf4 - TtCPN60 + CPN10 + ADP

Group II
3izh, 3izi, 3izj, 3izk, 3izl, 3izm, 3izn, 3los, 3iyf – MmCPN – Methanococcus maripaludis – EM<BR /> 3kfb, 3kfe, 3kfk – MmCPN<BR /> 1q2v, 1q3r – TkCPN α subunit (mutant) – Thermococcus KS-1<BR /> 1q3q - TkCPN α subunit (mutant) + AMP-PNP<BR /> 1q3s - TkCPN α subunit (mutant) + ADP

CCT

2xsm – bCPN CCT – bovine<BR /> 3iyg - bCPN CCT – Cryo EM<BR /> 3ktt - bCPN CCT β subunit – Cryo EM<BR /> 1gml, 1gn1 - CPN CCT γ subunit apical domain – mouse

Hsp33

3m7m, 1hw7 – EcCPN Hsp33 N-terminal<BR /> 1xjh - EcCPN Hsp33 C terminal - NMR<BR /> 1vq0 - CPN Hsp33 – Thermotoga maritime<BR /> 1vzy - CPN Hsp33 (mutant) – Bacillus subtilis

Thermosome

1a6d - TaTherm α+β subunits – Thermoplasma acidophilum<BR /> 1a6e - TaTherm α+β subunits + ADP<BR /> 1ass, 1asx - TaTherm α apical domain<BR /> 1e0r – TaTherm β apical domain

3ko1 – Therm – Acidianus tengchongensis 1lep – CPN-10 – Mycobacterium leprae